Sesquiterpene Synthases from Grand Fir (Abies grandis)
نویسندگان
چکیده
منابع مشابه
Monoterpene Synthases from Grand Fir (Abies grandis) cDNA ISOLATION, CHARACTERIZATION, AND FUNCTIONAL EXPRESSION OF MYRCENE SYNTHASE, (2)- (4S)-LIMONENE SYNTHASE, AND (2)-(1S,5S)-PINENE SYNTHASE*
Grand fir (Abies grandis) has been developed as a model system for studying defensive oleoresin formation in conifers in response to insect attack or other injury. The turpentine fraction of the oleoresin is a complex mixture of monoterpene (C10) olefins in which (2)limonene and (2)-aand (2)-b-pinene are prominent components; (2)-limonene and (2)-pinene synthase activities are also induced upon...
متن کاملMonoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.
Grand fir (Abies grandis) has been developed as a model system for studying defensive oleoresin formation in conifers in response to insect attack or other injury. The turpentine fraction of the oleoresin is a complex mixture of monoterpene (C10) olefins in which (-)-limonene and (-)-alpha- and (-)-beta-pinene are prominent components; (-)-limonene and (-)-pinene synthase activities are also in...
متن کاملTerpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible (E)-a-bisabolene synthase from grand fir (Abies grandis) [sesquiterpene synthaseyjuvenile hormone analogueyplant defense geney(E)-4-(1,5-dimethyl-1,4-hexadienyl)-1-methylcyclohexeneytodomatuic acid]
E)-a-Bisabolene synthase is one of two wound-inducible sesquiterpene synthases of grand fir (Abies grandis), and the olefin product of this cyclization reaction is considered to be the precursor in Abies species of todomatuic acid, juvabione, and related insect juvenile hormone mimics. A cDNA encoding (E)-a-bisabolene synthase was isolated from a wound-induced grand fir stem library by a PCR-ba...
متن کاملInsights into diterpene cyclization from the structure of the bifunctional abietadiene synthase from Abies grandis*
Insights into diterpene cyclization from the structure of the bifunctional abietadiene synthase from Abies grandis* Ke Zhou, Yang Gao, Julie A. Hoy, Francis M. Mann, Richard B. Honzatko and Reuben J. Peters From the Department of Biochemistry, Biophysics, & Molecular Biology, Iowa State University, Ames, IA 50011 USA Running title: Structure of abietadiene synthase Address correspondence to: Re...
متن کاملInsight into Biochemical Characterization of Plant Sesquiterpene Synthases
A fast and reproducible protocol was established for enzymatic characterization of plant sesquiterpene synthases that can incorporate radioactivity in their products. The method utilizes the 96-well format in conjunction with cluster tubes and enables processing of >200 samples a day. Along with reduced reagent usage, it allows further reduction in the use of radioactive isotopes and flammable ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.4.2078